ATLASINTESTINAL PARASITES (Protozoa)
      LOBOSEA Order: Amoebida

      ENTAMOEBA COLI

      coli1-ic

      Entamoeba coli: The identification of intestinal amoebae
      depends on the size and shape of trophozoites and cysts and
      on number of nuclei and aspect of karyosome and chromatin.
      E. coli is an harmless commensal of the colon with a world-wide distribution.

      coli2-ic

      By Dottoressa Vittoria Fabbrizi e gruppo di Batteriologia ospedale Civile di Teramo, Italy.

      coli_lugol1-ic Trophozoite Entamoeba coli Lugol 400X

       

      Entamoeba coli trophozoites measure 20-30 µm and have
      a vescicolous nucleus with a large eccentric karyosome
      and an irregulary distributed peripheral chromatin.
      The cytoplasm is vacuolated containing bacteria and yeast.
       

      Trophozoite Entamoeba coli Lugol 400X: Courtesy of Dottoressa Vittoria Fabbrizi e gruppo di Batteriologia ospedale Civile di Teramo, Italy.


      By Dr. Marc Lontie
      coli3-ic coliLeo-ic
      By Dottoressa Vittoria Fabbrizi e gruppo di Batteriologia ospedale Civile di Teramo, Italy.

      coli_lugol2-ic Entamoeba coli Lugol 400X

      By Dottoressa Vittoria Fabbrizi e gruppo di Batteriologia ospedale Civile di Teramo, Italy.

      coli_lugol3-ic Entamoeba coli Lugol-1000X

      E.coli cysts are spherical and measure 14-30 µm (usually 15-20).
      Mature cysts have 8 nuclei with a large karyosome
      (central or eccentric) and an irregular (sometimes regular) chromatin.
      The nuclei can be numerated with careful focusing.

      coliLeo-ic: Courtesy of  Dr. Marc Lontie:
      Director of the laboratory of the Medisch Centrum voor Huisartsen,
      Maria Theresiastraat 63a; B-3000 Leuven, Belgium.

      Entamoeba coli Lugol 400X and 1000X: Courtesy of Dottoressa Vittoria Fabbrizi e gruppo di Batteriologia ospedale Civile di Teramo, Italy.




      coli4-ic

      E.coli: with focusing three other nuclei are visible.



      By Daisy Zitova RNDr, Ph.D., Marek Bednar, MD, Ph.D. By Daisy Zitova RNDr, Ph.D., Marek Bednar, MD, Ph.D.

      coli5-ic                 coli6 

      Entamoeba coli: the spherical cysts are 15-20 µm in diameter. 
      Mature cysts contain 8 nuclei. 
      On both images cysts with 4 to 6 visible nuclei are present. 
      Stained fecal film. Wheatley modification of Gomori‘s trichrome technique. 
      Objective 100 X