INTESTINAL PARASITES (Protozoa)
LOBOSEA Order: Amoebida
ENTAMOEBA COLI
coli1-ic
Entamoeba coli: The
identification of intestinal amoebae
depends on the size and shape of trophozoites and cysts and
on number of nuclei and aspect of karyosome and chromatin.
E. coli is an harmless commensal of the colon with a
world-wide distribution.

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coli2-ic |
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coli_lugol1-ic
Trophozoite Entamoeba coli Lugol 400X |
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Entamoeba coli trophozoites
measure 20-30 µm and have
a vescicolous nucleus with a large eccentric karyosome
and an irregulary distributed peripheral chromatin.
The cytoplasm is vacuolated containing bacteria and yeast.
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Trophozoite Entamoeba coli Lugol 400X:
Courtesy of Dottoressa Vittoria
Fabbrizi e gruppo di
Batteriologia ospedale Civile di Teramo,
Italy. |

E.coli cysts are spherical and
measure 14-30 µm (usually 15-20).
Mature cysts have 8 nuclei with a large karyosome
(central or eccentric) and an irregular (sometimes regular) chromatin.
The nuclei can be numerated with careful focusing.
coliLeo-ic:
Courtesy of Dr. Marc Lontie:
Director of the laboratory of the
Medisch Centrum voor Huisartsen,
Maria Theresiastraat 63a; B-3000 Leuven, Belgium.
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Entamoeba coli Lugol
400X and 1000X:
Courtesy of Dottoressa Vittoria
Fabbrizi e gruppo di
Batteriologia ospedale Civile di Teramo,
Italy. |

coli4-ic
E.coli: with focusing three other
nuclei are visible.

coli5-ic
coli6
Entamoeba coli:
the spherical cysts are 15-20 µm in diameter.
Mature cysts contain 8 nuclei.
On both images cysts with 4 to 6 visible nuclei are present.
Stained fecal film. Wheatley modification of Gomori‘s trichrome technique.
Objective 100 X
 
 
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